Human plasma, barium citrate ppt. was separated on SDS-PAGE and probed with Monoclonal AB To Gelsolin (MO) CL:GS-2C4 . The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Antibody dilution 1/2,000.
Mouse monoclonal [GS - 2C4] to GelsolinGelsolin is a calcium dependent actin binding protein. It is a potent modulator of actin filament length and gelation. Gelsolin has been shown to exist in at least two variant forms, cytoplasmic gelsolin and plasma gelsolin. Plasma gelsolin has also been called actin depolymerizing factor (ADF) or brevin. Human plasma and rabbit macrophage gelsolins differ by the presence of a 25 amino acid residue extension at the NH2 terminus in the human plasma gelsolin which appears to account for the difference in relative molecular weights.
Immunogen
Full length native protein (purified) (Human).
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
GS-2C4
Host Species
Mouse
Tested Applications
PurifyELISAICC/IFWB
AP: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Predicted molecular weight: 86 kDa. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity
HumanPigRabbitBovine
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
P06396 GSN 2934 DKFZp313L0718 105120 Gelsolin
Target
Gelsolin
Cellular Localization
Cytoplasmic and Secreted
Application
Application
Human plasma, barium citrate ppt. was separated on SDS-PAGE and probed with Monoclonal AB To Gelsolin (MO) CL:GS-2C4 . The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Antibody dilution 1/2,000.
Application Notes
AP: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Predicted molecular weight: 86 kDa. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.