Application
WB analysis of 293T lysates (50ug) using Geminin antibody at a dilution of 1:500.| Product Name | Geminin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2H7] to GemininThis gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011] |
| Immunogen | Recombinant human Geminin (1-209 aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2H7 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GMNN geminin DNA replication inhibitor Gem |
|---|---|
| Target | Geminin |
| Light Chain Type | kappa |
Application
WB analysis of 293T lysates (50ug) using Geminin antibody at a dilution of 1:500.| Application Notes | The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
