Application
IHC Image using - Detection of GFAP by IHC in Human BrainApplication
Formalin fixed paraffin embedded human brain stained with GFAP antibody (cat. # ).| Product Name | GFAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Porcine Glial Fibrillary Acidic Protein ( GFAP )This antibody reacts with the 52 kD intermediate filament protein GFAP in brain and spinal cord. It labels some astrocytes and some CNS ependymal cells but not oligodendrocytes or neurons. This antibody does not react with other intermediate filament proteins. |
| Immunogen | BALB/C mice were immunized with glial fibrillary protein from porcine spinal cord. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GA5 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PIPWB |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Can also be used for: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AI836096 |
|---|---|
| Target | GFAP |
| Cellular Localization | Cytoplasm |
| Light Chain Type | Kappa |
Application
IHC Image using - Detection of GFAP by IHC in Human Brain
Application
Formalin fixed paraffin embedded human brain stained with GFAP antibody (cat. # ).| Positive Control | Brain |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Can also be used for: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100): |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | PBS containing 0.05% sodium azide |
