| Product Name | GFAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [GF5] to GFAP - Astrocyte MarkerGlial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. |
| Immunogen | This clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with purified glial fibrillary acidic protein from human brain. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GF5 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC |
| ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | P14136 137780 FLJ45472 2670 GFAP |
|---|---|
| Target | GFAP |
| Tissue Specificity | There is no cross-reactivity with other neurospecific proteins. |
| Cellular Localization | Cytoplasmic |
| Application Notes | ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide |
