Application
Western blot detection of GFP-MIP2 fusion protein in transfectant by anti-GFP.| Product Name | GFP antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Rabbit polyclonal to GFPThe jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells. |
| Immunogen | Purified protein - native GFP Aequorea victoria |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| For IP: Use at a dilution of 1:200-1:500. For WB: Use at a dilution of 1:5,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | Green Fluorescent Protein |
|---|---|
| Target | GFP |
| Tissue Specificity | This antibody reacts with wild-type GFP, and itÕs variants, such as EGFP, EBFP. |
Application
Western blot detection of GFP-MIP2 fusion protein in transfectant by anti-GFP.| Application Notes | For IP: Use at a dilution of 1:200-1:500. For WB: Use at a dilution of 1:5,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH7.2 containing 0.1% sodium azide |
