| Product Name | GFP antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Rabbit polyclonal to GFPA highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos. |
| Immunogen | Highly purified recombinant full length protein made in Escherichia coli. The antibody is directed against the entire GFP molecule. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | EMICC/IFIHCIHC-PIPWB |
| For EM (Immunogold): Use at a dilution of 1:100. For ICC: Use at a dilution of 1:200-1:1,000. For IHC-P: Use at a dilution of 1:200. Perform heat mediated antigen retrieval using microwave method. For IP: Use 1 μl crude serum per 10cm tissue culture dish of transfected cells. Use 10 μl protein A agarose CL4B to precipitate immune complex. For WB: Use at a dilution of 1:2,500-1:10,000. Use in 1x BLOTTO or 3% BSA in PBS. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Green Fluorescent Protein |
|---|---|
| Target | GFP |
| Tissue Specificity | Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP. |
| Positive Control | Detects 5ng of recombinant GFP (using ECL or ECL Plus) in under one minute of exposure to film. |
|---|---|
| Application Notes | For EM (Immunogold): Use at a dilution of 1:100. For ICC: Use at a dilution of 1:200-1:1,000. For IHC-P: Use at a dilution of 1:200. Perform heat mediated antigen retrieval using microwave method. For IP: Use 1 μl crude serum per 10cm tissue culture dish of transfected cells. Use 10 μl protein A agarose CL4B to precipitate immune complex. For WB: Use at a dilution of 1:2,500-1:10,000. Use in 1x BLOTTO or 3% BSA in PBS. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.02M KPO4, 0.15M NaCl, pH 7.2, containing 0.01% sodium azide |
