Application
GFP antibody detects GFP protein by western blot analysis. A. 1 µg 293T whole cell extract B. 1 µg 293T whole cell extract expressing GFP-tagged protein C. 1 µg 293T whole cell extract expressing RFP-tagged protein 12% SDS-PAGE GFP antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo, using GFP antibody at 1:200 dilution.Application
IP-WB assay to show that Hice1 co-immunoprecipitated with other Augmin components HAUS6 , HAUS2 and HAUS1 in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.Application
GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody . The precipitated GFP was detected by diluted at 1:15000.Application
GFP antibody detects GFP protein by western blot analysis. Non-transfected (-) and GFP-transfected (+, ) 293T whole cell extracts (1 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | GFP antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Rabbit Polyclonal antibody to GFP (Green Fluorescent Protein) |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-FIHC-PIHC-WIPWB |
| WB:1:1000~1:10000 IP:1:100~1:500 ICC/IF:1:50~1:100 IHC:1:50~1:200 |
|
| Species Reactivity | Other |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Green Fluorescent Protein GFP eGFP enhanced green fluorescent protein |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) |
| Tissue Specificity | This antibody reacts with GFP variants including GFP, EGFP, and YFP. |
Application
GFP antibody detects GFP protein by western blot analysis. A. 1 µg 293T whole cell extract B. 1 µg 293T whole cell extract expressing GFP-tagged protein C. 1 µg 293T whole cell extract expressing RFP-tagged protein 12% SDS-PAGE GFP antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo, using GFP antibody at 1:200 dilution.
Application
IP-WB assay to show that Hice1 co-immunoprecipitated with other Augmin components HAUS6 , HAUS2 and HAUS1 in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.
Application
GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody . The precipitated GFP was detected by diluted at 1:15000.
Application
GFP antibody detects GFP protein by western blot analysis. Non-transfected (-) and GFP-transfected (+, ) 293T whole cell extracts (1 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | human GFP-transfected 293T , 293T whole cell extract expressing GFP-tagged protein , *GFP-p53 transfected U2OS |
|---|---|
| Application Notes | WB:1:1000~1:10000 IP:1:100~1:500 ICC/IF:1:50~1:100 IHC:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
