WB
Western blot analysis of lysates from insect cells transfected with GFP using Rabbit anti GFP-Tag mAb at 1:10000-1:2560000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 20ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.Exposure time: 3s.ICC/IF
Immunofluorescence analysis of 293T and 293T-GFP cells using Rabbit anti GFP-Tag mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.WB
(C) The expression of affbody-GFP was verified by Western Blot analysis at the level of translation. AE-Mφ was compared with RAW264.7 macrophage (Mφ) in which β-actin was used for standardization for each sample.WB
(G) HER2 protein was incubated with AE-Mφ or Mφ to obtain the input sample. The eluent products as CO-IP samples of AE-Mφ immunoprecipitated along with anti-GFP indicates a direct interaction between AE-Mφ and anti-GFP antibody with the commercial resin column, No protein were found on the output samples.| Product Name | Rabbit anti GFP-Tag mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-238 of GFP. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:50000-1:700000 ICC:1:50-1:200 |
|
| Species Reactivity | A wide range of other species |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GFP |
|---|---|
| Alternative Names | GFP GFP tag GFP-tag |
| Molecular Weight(MW) | 27kDa |
WB
Western blot analysis of lysates from insect cells transfected with GFP using Rabbit anti GFP-Tag mAb at 1:10000-1:2560000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 20ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.Exposure time: 3s.
ICC/IF
Immunofluorescence analysis of 293T and 293T-GFP cells using Rabbit anti GFP-Tag mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
WB
(C) The expression of affbody-GFP was verified by Western Blot analysis at the level of translation. AE-Mφ was compared with RAW264.7 macrophage (Mφ) in which β-actin was used for standardization for each sample.
WB
(G) HER2 protein was incubated with AE-Mφ or Mφ to obtain the input sample. The eluent products as CO-IP samples of AE-Mφ immunoprecipitated along with anti-GFP indicates a direct interaction between AE-Mφ and anti-GFP antibody with the commercial resin column, No protein were found on the output samples.| Application Notes | WB:1:50000-1:700000 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
