WB
HEK293 cells, CHO cells, normal mouse brain and GFP transgenic mouse brain were subjected to SDS PAGE followed by western blot with GFP tag antibody at dilution of 1:200000 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag antibody at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).| Product Name | GFP tag Mouse Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. |
| Immunogen | Recombinant aequorea victoria GFP tag protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:20000-1:100000 ICC:1:400-1:1600 |
|
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GFP |
|---|---|
| Alternative Names | eGFP GFP CFP eYFP GFP-tag |
| Molecular Weight(MW) | 27kDa |
WB
HEK293 cells, CHO cells, normal mouse brain and GFP transgenic mouse brain were subjected to SDS PAGE followed by western blot with GFP tag antibody at dilution of 1:200000 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag antibody at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:20000-1:100000 ICC:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
