Application
1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1μg (2) and 10μg (3) of cell lysates used| Product Name | GFP antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal antibody [GF28R] to GFPA highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos. |
| Immunogen | GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GF28R |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIPWB |
| WB (with ECL): Use at a dilution of 1:1000-3000 : |
|
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | eGFP Green Fluorescent Protein GFP GFP Tag Enhanced Green Fluorescent Protein |
|---|---|
| Target | GFP |
| Tissue Specificity | Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP |
Application
1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1μg (2) and 10μg (3) of cell lysates used| Application Notes | WB (with ECL): Use at a dilution of 1:1000-3000 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. Avoid repeated freeze-thaw cycles |
| Storage Buffer | 10mM PBS, pH 7.2 |
