Application
ELISA detection of GFP using for capture and for detection. Coating Ab: mouse anti-GFP (, 2µg/well ) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP ( 1:20000)Application
GFP antibody detects GFP protein by western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg 293T whole cell extract expressing GFP-tagged protein C. 30 µg 293T whole cell extract expressing RFP-tagged protein 12% SDS-PAGE GFP antibody dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.| Product Name | GFP antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse Monoclonal antibody to GFP (Green Fluorescent Protein) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT7312 |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Molecular Weight(MW) | 29 kDa (note) |
|---|---|
| Tissue Specificity | This antibody reacts with GFP variants including GFP, EGFP, and YFP. |
Application
ELISA detection of GFP using for capture and for detection. Coating Ab: mouse anti-GFP (, 2µg/well ) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP ( 1:20000)
Application
GFP antibody detects GFP protein by western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg 293T whole cell extract expressing GFP-tagged protein C. 30 µg 293T whole cell extract expressing RFP-tagged protein 12% SDS-PAGE GFP antibody dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.| Positive Control | 293T whole cell extract expressing GFP-tagged protein |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
