Application
Antigen retrieval was done with Tris-EDTA, pH9.0 in pressure cooker for 20 minutes. Optimum staining of L-type cells secreting GLP1 was observed.
Application
Anti-human GLP1 antibody was used at 1 µg per well for detection of antigen (peptide, 1µg serially diluted and coated). The antibody at 10 µg/ml concentration can sensitively detect coated antigen up to 2 ng per well.