Application
The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 μl IP reaction solution) using 5 μg of Glucocorticoid receptor antibody. The immunoprecipitated proteins were analysed by WB with Glucocorticoid receptor antibody.Application
IHC analysis of rat CA1 neurons of hippocampus using Glucocorticoid receptor antibody at a concentration of 2.5 μg/ml.Application
ICC analysis of COS-7 cells transiently overexpressing hGR using Glucocorticoid receptor antibody at a concentration of 2.5 μg/ml.Application
WB analysis of extracts from (A) HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), Raji and Molt cells and from (B) HeLa cells using Glucocorticoid receptor antibody.Application
Sandwich ELISA was assessed using Glucocorticoid receptor antibody against recombinant hGR at a concentration of 0.5 μg/μl.| Product Name | Glucocorticoid receptor antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to Glucocorticoid receptor |
| Clonality | Monoclonal |
|---|---|
| Clone Number | GT9006 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSGSAICC/IFIHCIPWBChIP |
| ChIP (ChIP-on-chip) (5 µg/IP), ELISA (0.5 µg/ml), Western blotting (1 µg/ml), Gel Supershift (5 µg/ml), Immunochemistry (2.5 µg/ml), Flow cytometry (0.5 µg/ml), Immunoprecipitation (5 µg per IP): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | GR |
|---|
Application
The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 μl IP reaction solution) using 5 μg of Glucocorticoid receptor antibody. The immunoprecipitated proteins were analysed by WB with Glucocorticoid receptor antibody.
Application
IHC analysis of rat CA1 neurons of hippocampus using Glucocorticoid receptor antibody at a concentration of 2.5 μg/ml.
Application
ICC analysis of COS-7 cells transiently overexpressing hGR using Glucocorticoid receptor antibody at a concentration of 2.5 μg/ml.
Application
WB analysis of extracts from (A) HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), Raji and Molt cells and from (B) HeLa cells using Glucocorticoid receptor antibody.
Application
Sandwich ELISA was assessed using Glucocorticoid receptor antibody against recombinant hGR at a concentration of 0.5 μg/μl.| Application Notes | ChIP (ChIP-on-chip) (5 µg/IP), ELISA (0.5 µg/ml), Western blotting (1 µg/ml), Gel Supershift (5 µg/ml), Immunochemistry (2.5 µg/ml), Flow cytometry (0.5 µg/ml), Immunoprecipitation (5 µg per IP): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.1% BSA |
