Application
Western blot of glucocorticoid receptor in mouse liver extract.Application
Immunofluorescent analysis of Glucocorticoid Receptor using in HeLa cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Glucocorticoid Receptor antibody [BuGR2] at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.Application
Flow cytometry analysis of Glucocorticoid Receptor in HeLa cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant, adding 90% methanol and incubated for 10 minutes at room temperature. Follwing penetration, cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with Glucocorticoid Receptor antibody [BuGR2] at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a proper secondary antibody and re-suspended in PBS for FACS analysis.| Product Name | Glucocorticoid Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [BuGR2] to Glucocorticoid Receptorglucocorticoid receptor that binds and activates hormone-dependent transcriptional enhancers [RGD, Feb 2006] |
| Immunogen | Partially purified rat GR. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | BuGR2 |
| Host Species | Mouse |
| Tested Applications | BlockingELISAFACSGSAICC/IFIHCIHC-PIPWBChIP |
| IHC-P: 5 µg/ml: |
|
| Species Reactivity | HumanMouseRatYeastGuinea PigPrimateRabbitSheepXenopusInvertebrates |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | GR nuclear receptor subfamily 3 group C member 1 Gcr Nr3c1 Grl |
|---|---|
| Target | Glucocorticoid Receptor |
Application
Western blot of glucocorticoid receptor in mouse liver extract.
Application
Immunofluorescent analysis of Glucocorticoid Receptor using in HeLa cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Glucocorticoid Receptor antibody [BuGR2] at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.
Application
Flow cytometry analysis of Glucocorticoid Receptor in HeLa cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant, adding 90% methanol and incubated for 10 minutes at room temperature. Follwing penetration, cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with Glucocorticoid Receptor antibody [BuGR2] at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a proper secondary antibody and re-suspended in PBS for FACS analysis.| Application Notes | IHC-P: 5 µg/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS containing 0.05 % sodium azide |
