Application
Western blot analysis of extract from mouse brain tissue, using Glutamate receptor 2 (Precursor) antibody (Lane 1 and 2) and Glutamate receptor 2 (Precursor)(phospho-Ser880) antibody (Lane 3 and 4).| Product Name | GluR2 (phospho Ser880) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GluR2 (phospho S880)Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human GluR2 around the phosphorylation site of serine 880 (I-E-pS-V-K). |
| Modification | p-Ser880 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GLUR2 GLURB HBGR2 |
|---|---|
| Target | GluR2 (phospho Ser880) |
Application
Western blot analysis of extract from mouse brain tissue, using Glutamate receptor 2 (Precursor) antibody (Lane 1 and 2) and Glutamate receptor 2 (Precursor)(phospho-Ser880) antibody (Lane 3 and 4).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS (without Mg2+and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. |
