Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Glutathione S-transferase kappa 1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 12% SDS PAGE diluted at 1:1000Application
Glutathione S-transferase kappa 1 antibody detects GSTK1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GSTK1 protein stained by Glutathione S-transferase kappa 1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.| Product Name | Glutathione S-transferase kappa 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Glutathione S-transferase kappa 1 (glutathione S-transferase kappa 1)This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glutathione S-transferase kappa 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (83%), Rhesus Monkey (92%), Bovine (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GST antibody GST 13-13 antibody GST13 antibody GST13-13 antibody GSTK1-1 antibody hGSTK1 antibody GSTK1 antibody glutathione S-transferase kappa 1 antibody GST class-kappa antibody glutathione S-transferase k1 antibody glutathione S-transferase s |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) (3) |
| Cellular Localization | Peroxisome |
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Glutathione S-transferase kappa 1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 12% SDS PAGE diluted at 1:1000
Application
Glutathione S-transferase kappa 1 antibody detects GSTK1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GSTK1 protein stained by Glutathione S-transferase kappa 1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.| Positive Control | 293T , A431 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
