Application
Recombinant GST protein(20ng,Lane1) and 293 cellular lysates transfected with a GST expression vector(20μg,Lane2) were probed with anti-GST antibody(1μg/ml).Arrow indicates GST(~26kDa) and GST-fusion protein(~29kDa).| Product Name | GST antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal [GST.B6] to GST |
| Immunogen | Anti-GST antibody was produced by immunizing mice with a GST peptide. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GST.B6 |
| Host Species | Mouse |
| Tested Applications | IHCIPWB |
| IHC: 1/200. IP: Use at an assay dependent dilution. WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | GST antibody |
|---|---|
| Target | GST |
| Tissue Specificity | GST-tag monoclonal antibody detects over expressed glutathione- transferase (GST) fusion proteins. |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Recombinant GST protein(20ng,Lane1) and 293 cellular lysates transfected with a GST expression vector(20μg,Lane2) were probed with anti-GST antibody(1μg/ml).Arrow indicates GST(~26kDa) and GST-fusion protein(~29kDa).| Application Notes | IHC: 1/200. IP: Use at an assay dependent dilution. WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
