Application
Formalin fixed paraffin embedded human tonsils stained with glycophorin A monoclonal antibody (cat. # ).| Product Name | Glycophorin A antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [JC159] to Glycophorin A predilutedGlycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. |
| Immunogen | BALB/C mice were immunized with formalin fixed membrane from a Hairy cell leukemia. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | JC159 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | Glycophorin A GPSAT HGpMiX HGpSta(C) 14934 111300 MNS Gypa HGpMiXI P02724 HGpMiV GPA CD235a MN AI853584 2993 HGpMiIII GPErik GpMiIII |
|---|---|
| Target | Glycophorin A |
| Tissue Specificity | This antibody is specific to erythroid cells at various stages of differentiation from the erythroblast to the mature red cell. |
| Cellular Localization | Cell Membrane |
| Light Chain Type | kappa |
Application
Formalin fixed paraffin embedded human tonsils stained with glycophorin A monoclonal antibody (cat. # ).| Positive Control | Tonsil |
|---|---|
| Application Notes | IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 2-8°C. |
| Storage Buffer | Constituents: PBS |
