Application
Sample(30 μg of whole cell lysate) A:A431 B:Hep G2 7.5% SDS PAGE diluted at 1:500Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using GLYCTK antibody at 1:100 dilution.Application
GLYCTK antibody [N3C3] detects GLYCTK protein at cytoplasm by confocal immunofluorescent analysis. Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: GLYCTK protein stained by GLYCTK antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]| Product Name | GLYCTK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GLYCTK (glycerate kinase)This locus encodes a member of the glycerate kinase type-2 family. The encoded enzyme catalyzes the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. Decreased activity of the encoded enzyme may be associated with the disease D-glyceric aciduria. Alternatively spliced transcript variants have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GLYCTK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (80%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HBEBP2 antibody, HBEBP4 antibody, HBeAgBP4A antibody, GLYCTK antibody, HBeAg binding protein 4 antibody, glycerate kinase antibody, HBeAg-binding protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (2) |
| Cellular Localization | Isoform 1: Cytoplasm , Isoform 2: Cytoplasm , Mitochondrion |
Application
Sample(30 μg of whole cell lysate) A:A431 B:Hep G2 7.5% SDS PAGE diluted at 1:500
Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using GLYCTK antibody at 1:100 dilution.
Application
GLYCTK antibody [N3C3] detects GLYCTK protein at cytoplasm by confocal immunofluorescent analysis. Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: GLYCTK protein stained by GLYCTK antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]| Positive Control | A431 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
