Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [GT266] diluted at 1:1000.Application
Glyoxalase I antibody [GT266] detects GLO1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract D. 30 µg A375 whole cell lysate/extract 12 % SDS-PAGE Glyoxalase I antibody [GT266] dilution: 1:1000| Product Name | Glyoxalase I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to Glyoxalase I (glyoxalase I)The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT266 |
| Host Species | Mouse |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Rhesus Monkey, BovineMouse (92%), Rat (91%), Xenopus laevis (82%), Rhesus Monkey (97%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 21 kDa (note) |
|---|
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [GT266] diluted at 1:1000.
Application
Glyoxalase I antibody [GT266] detects GLO1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract D. 30 µg A375 whole cell lysate/extract 12 % SDS-PAGE Glyoxalase I antibody [GT266] dilution: 1:1000| Positive Control | 293T , HeLa , HepG2 , A375 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
