Application
Glypican-3 antibody [GT2473] detects Glypican-3 protein at cell membrane by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glypican-3 protein stained by Glypican-3 antibody [GT2473] diluted at 1:250. Blue: Hoechst 33342 staining.Application
Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm in human hepatocellular carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. Glypican-3 antibody [GT2473] diluted at 1:100.Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT2473] diluted at 1:1000.| Product Name | Glypican-3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT2473] to Glypican-3 (glypican 3)Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT2473 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Chimpanzee, BovineChimpanzee (96%), Bovine (81%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DGSX antibody GPC3 antibody GTR22 antibody Glypican-3 antibody [GT2473] antibody Glypican-3 antibody MXR7 antibody OCI5 antibody SDYS antibody SGB antibody SGBS antibody SGBS1 antibody glypican 3 antibody |
|---|---|
| Target | Glypican-3 |
| Molecular Weight(MW) | 66 kDa (note) (4) |
| Cellular Localization | Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-3: Secreted , extracellular space |
Application
Glypican-3 antibody [GT2473] detects Glypican-3 protein at cell membrane by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glypican-3 protein stained by Glypican-3 antibody [GT2473] diluted at 1:250. Blue: Hoechst 33342 staining.
Application
Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm in human hepatocellular carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. Glypican-3 antibody [GT2473] diluted at 1:100.
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT2473] diluted at 1:1000.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
