Application
Anti-GORASP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GORASP1Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GORASP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GORASP1.| Product Name | GORASP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4F8] to GORASP1Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes. |
| Immunogen | Full length human recombinant protein of human GORASP1 (NP_114105) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 4F8 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF 1:100,: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GRASP65 GORASP1 P65 GOLPH5 FLJ23443 MGC118894 MGC118897 |
|---|---|
| Target | GORASP1 |
| Tissue Specificity | is specific for human GORASP1 (NP_114105). |
| Cellular Localization | Undergoes rapid exchange with the cytosol. |
Application
Anti-GORASP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GORASP1
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GORASP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GORASP1.| Application Notes | ICC/IF 1:100,: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
