Application
GPA33 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
GPA33 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPA33 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of GPA33 Antibody (C-term) in K562, 293, MDA-MB231 cell line lysates (35ug/lane). GPA33 (arrow) was detected using the purified Pab.| Product Name | GPA33 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to GPA33 (C-term)The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 271~301 amino acids from the C-terminal region of human GPA33. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GPA33 Glycoprotein A33 Cell surface A33 antigen |
|---|---|
| Target | GPA33 |
| Molecular Weight(MW) | 35.632 kDa (note) |
Application
GPA33 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
GPA33 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPA33 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of GPA33 Antibody (C-term) in K562, 293, MDA-MB231 cell line lysates (35ug/lane). GPA33 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
