Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
GPA33 Antibody IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35ug/lane)Application
Western Blot at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | GPA33 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cell surface A33 antigen, Glycoprotein A33, GPA33 |
| Product description | GPA33 is a cell surface antigen that is expressed in greater than 95% of human colon cancers. It may play a role in cell-cell recognition and signaling.1) Moritz,R.L., et.al.,Chromatogr798 (1-2), 91-101 (1998) |
| Immunogen | This GPA33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the C-terminal region of human GPA33. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPA33 |
|---|---|
| Alternative Names | Cell surface A33 antigen Glycoprotein A33 GPA33 |
| Molecular Weight(MW) | 36 kDa |
Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
GPA33 Antibody IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35ug/lane)
Application
Western Blot at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
