Application
Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5 ug/ml.Application
Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 ug/ml.Application
Immunofluorescence of GPAT1 in Rat Brain cells with GPAT1 antibody at 20 ug/mL.| Product Name | GPAT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GPAT1Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets. At least two isoforms of GPAT1 are known to exist. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Glycerol3phosphate acyltransferase1 GPAM Glycerol3phosphate acyltransferase 1 GPAT1 Glycerol-3-phosphate acyltransferase 1 |
|---|---|
| Target | GPAT1 |
| Tissue Specificity | This antibody is specific for GPAT1 |
Application
Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5 ug/ml.
Application
Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 ug/ml.
Application
Immunofluorescence of GPAT1 in Rat Brain cells with GPAT1 antibody at 20 ug/mL.| Positive Control | Blocking Peptide: Cat.No. GTX85034-PEP - GPAT1 Peptide Lysates: Cat. No. GTX27942 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
