Application
WB analysis of (1) recombinant human GPC4 (50ng) and (2) Ramos lysate (40ug) using GPC4 antibody [AT51E3] at a dilution of 1:1000.Application
FACS analysis of 293T cells using an isotype control (black) or GPC4 antibody [AT51E3] at 2-5ug for 1,000,000 cells (red).Application
ICC/IF analysis of 293T cells using DAPI (blue) and GPC4 antibody [AT51E3] at a dilution of 1:200 (green).| Product Name | GPC4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [AT51E3] to GPC4Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant human GPC4 (401-529aa) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | AT51E3 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| WB: 1:1000. ICC/IF: 1:200. FACS: 1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | K-glypican glypican 4 |
|---|---|
| Target | GPC4 |
| Light Chain Type | kappa |
Application
WB analysis of (1) recombinant human GPC4 (50ng) and (2) Ramos lysate (40ug) using GPC4 antibody [AT51E3] at a dilution of 1:1000.
Application
FACS analysis of 293T cells using an isotype control (black) or GPC4 antibody [AT51E3] at 2-5ug for 1,000,000 cells (red).
Application
ICC/IF analysis of 293T cells using DAPI (blue) and GPC4 antibody [AT51E3] at a dilution of 1:200 (green).| Application Notes | WB: 1:1000. ICC/IF: 1:200. FACS: 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -32C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
