Application
Confocal immunofluorescence analysis of L-02 cells using GPI mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin Blue: DRAQ5 fluorescent DNA dyeApplication
Immunohistochemical analysis of paraffin-embedded human kidney tissues using GPI mouse mAb.Application
Western blot analysis using GPI mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GPI cDNA (2).Application
Confocal immunofluorescence analysis of L-02 cells using anti-GPI mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Application
Western blot analysis using anti-GPI mAb against HepG2 (1) , SMMC-7721 (2) cell lysate and rat liver tissues lysate (3).| Product Name | GPI antibody [1B7D7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal Antiobdy [1B7D7] to Glucose-6-phosphate isomeraseThis gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human GPI expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1B7D7 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ELISA: 1/10000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GNPI , SA-36 , SA36 , AMF , PHI , PGI , NLK |
|---|---|
| Target | Glucose-6-phosphate isomerase |
| Molecular Weight(MW) | 63 kDa (note) |
Application
Confocal immunofluorescence analysis of L-02 cells using GPI mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin Blue: DRAQ5 fluorescent DNA dye
Application
Immunohistochemical analysis of paraffin-embedded human kidney tissues using GPI mouse mAb.
Application
Western blot analysis using GPI mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GPI cDNA (2).
Application
Confocal immunofluorescence analysis of L-02 cells using anti-GPI mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Application
Western blot analysis using anti-GPI mAb against HepG2 (1) , SMMC-7721 (2) cell lysate and rat liver tissues lysate (3).| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
