Application
Anti-GPI mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GPIApplication
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GPI mouse monoclonal antibody. ( Dilution 1:50)Application
Flow cytometric Analysis of Jurkat cells, using anti-GPI antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPI .Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPI monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-GPI mouse monoclonal antibody. ( Dilution 1:50)| Product Name | GPI antibody [5A11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5A11] to GPIBesides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. |
| Immunogen | Full length human recombinant protein of human GPI(NP_000166) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 5A11 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 | |
| Species Reactivity | HumanDogMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NLK, GPI, GNPI, AMF, PHI, SA36, PGI, SA-36, SA 36 |
|---|---|
| Target | GPI |
| Tissue Specificity | is specific for human GPI(NP_000166). |
Application
Anti-GPI mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GPI
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GPI mouse monoclonal antibody. ( Dilution 1:50)
Application
Flow cytometric Analysis of Jurkat cells, using anti-GPI antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPI .
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPI monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-GPI mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
