Application
WB analysis of HeLa lysate (100ug) using GPS2 antibody at a dilution of 1:500.| Product Name | GPS2 antibody [1E2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1E2] to GPS2This gene encodes a protein involved in G protein-mitogen-activated protein kinase (MAPK) signaling cascades. When overexpressed in mammalian cells, this gene could potently suppress a RAS- and MAPK-mediated signal and interfere with JNK activity, suggesting that the function of this gene may be signal repression. The encoded protein is an integral subunit of the NCOR1-HDAC3 (nuclear receptor corepressor 1-histone deacetylase 3) complex, and it was shown that the complex inhibits JNK activation through this subunit and thus could potentially provide an alternative mechanism for hormone-mediated antagonism of AP1 (activator protein 1) function. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant human GPS2 (161-311aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | 1E2 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | G protein pathway suppressor 2 , AMF-1 |
|---|---|
| Target | GPS2 |
| Light Chain Type | kappa |
Application
WB analysis of HeLa lysate (100ug) using GPS2 antibody at a dilution of 1:500.| Application Notes | The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
