| Product Name | Gram negative endotoxin antibody [308] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [308] to Gram Negative EndotoxinEndotoxins are part of the outer membrane of the cell wall of gram-negative bacteria. Endotoxins are invariably associated with gram-negative bacteria whether the organisms are pathogens or not. Although the term "endotoxin" is occasionally used to refer to any cell-associated bacterial toxin, it is properly reserved to refer to the lipopolysaccharide complex associated with the outer membrane of gram-negative bacteria such as E. coli, Salmonella, Shigella, Pseudomonas, Neisseria, Haemophilus, and other leading pathogens.The biological activity of endotoxin is associated with the lipopolysaccharide (LPS). Toxicity is associated with the lipid component (Lipid A) and immunogenicity is associated with the polysaccharide components. The cell wall antigens (O antigens) of gram-negative bacteria are components of LPS. LPS elicits a variety of inflammatory responses in an animal. Because it activates complement by the alternative (properdin) pathway, it is often part of the pathology of gram-negative bacterial infections. |
| Immunogen | Full length protein (E. coli). E. coli O:111 B4 J5 cells, Ascites. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | 308 |
| Host Species | Mouse |
| Tested Applications | IA |
| IA: Use at an assay dependent dilution.May be used in immunoassays to detect and quantitate gram negative endotoxin or cells that express endotoxin. Dilute in PBS or medium which is identical to that used in the assay system. Optimal dilutions/concentrations should be determined by the end user. | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Gram negative endotoxin |
|---|---|
| Cellular Localization | Cell Membrane |
| Light Chain Type | kappa |
| Application Notes | IA: Use at an assay dependent dilution.May be used in immunoassays to detect and quantitate gram negative endotoxin or cells that express endotoxin. Dilute in PBS or medium which is identical to that used in the assay system. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide |
