Application
Formalin-fixed, paraffin-embedded human tonsil stained with using peroxidase-conjugate and AEC chromogen. Note nuclear and cytoplasmic staining of cell.| Product Name | GRB2 antibody [2GB04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2GB04] to GRB2GRB 2 protein contains on SH2 domain flanked on both sides by an SH3 domain. This antibody binds to the junction between the amino terminal SH3 domain and SH2 domain of GRB 2. This antibody binds to GRB 2 at its epitope maps between amino acids 54-62. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex then binds the EGF-receptor. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2GB04 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| Immunohistochemistry (formalin fixed paraffin embedded / frozen sections): use at a dliution of 1/50 - 1/75 in an ABC method. Optimal dilutions / concentrations should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. | |
| Species Reactivity | HumanMouseRatXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | ASH, MST084, MSTP084, Grb33, EGFRBPGRB2, 2885, 108355, EGFRBP-GRB2, P62993, Grb3-3, GRB2, EGFRBP GRB2 |
|---|---|
| Target | GRB2 |
| Tissue Specificity | This antibody reacts with a 24 - 25 kDa protein. |
| Cellular Localization | Cytoplasmic (probable) |
| Light Chain Type | kappa |
Application
Formalin-fixed, paraffin-embedded human tonsil stained with using peroxidase-conjugate and AEC chromogen. Note nuclear and cytoplasmic staining of cell.| Positive Control | Suitable tissue control sections for Immunohistochemistry:Tonsil |
|---|---|
| Application Notes | Immunohistochemistry (formalin fixed paraffin embedded / frozen sections): use at a dliution of 1/50 - 1/75 in an ABC method. Optimal dilutions / concentrations should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Liquid with sodium azide |
