Application
WB analysis of various cell lysates using Grp78 antibody [9E4-2A7-H6] at a dilution of 1:1000.Application
ICC/IF analysis of HeLa cells using Grp78 antibody [9E4-2A7-H6] at a dilution of 1:50.Application
IHC-P analysis of colorectal cancer using Grp78 antibody [9E4-2A7-H6] at a dilution of 1:100. Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).| Product Name | Grp78 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [9E4-2A7-H6] to Grp78The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010] |
| Immunogen | Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 9E4-2A7-H6 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GRP78 BIP HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein 78kDa) MIF2 |
|---|---|
| Target | Grp78 |
Application
WB analysis of various cell lysates using Grp78 antibody [9E4-2A7-H6] at a dilution of 1:1000.
Application
ICC/IF analysis of HeLa cells using Grp78 antibody [9E4-2A7-H6] at a dilution of 1:50.
Application
IHC-P analysis of colorectal cancer using Grp78 antibody [9E4-2A7-H6] at a dilution of 1:100. Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stored at -20°C |
| Storage Buffer | PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol. |
