Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Grp78 antibody [N2C1], Internal detects Grp78 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody [N2C1], Internal diluted at 1:100. Blue: Hoechst 33342 staining.Application
Immunoprecipitation of Grp78 protein from HepG2 whole cell extracts using 5 μg of Grp78 antibody [N2C1], Internal . Western blot analysis was performed using Grp78 antibody [N2C1], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Grp78 antibody [N2C1], Internal dilution: 1:50.Application
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Grp78 antibody [N2C1], Internal dilution: 1:50.Application
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Grp78 antibody [N2C1], Internal diluted at 1:50.Application
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. Grp78 antibody [N2C1], Internal diluted at 1:50.Application
Grp78 antibody detects Grp78 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at a dilution of 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Grp78 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Grp78 (heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa))When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| WB:1:100~1:400 IP:1:20~1:100 ICC/IF:1:20~1:100 IHC-p:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Japanese medaka, Xenopus laevis, Chicken, Rhesus Monkey, BovineZebrafish (98%), Japanese medaka (97%), Xenopus laevis (97%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BIP antibody FLJ26106 antibody GRP78 antibody MIF2 antibody HSPA5 antibody immunoglobulin heavy chain-binding protein antibody 78 kDa glucose-regulated protein antibody endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody |
|---|---|
| Target | Grp78 |
| Molecular Weight(MW) | 72 kDa (note) |
| Tissue Specificity | Knockdown/Knockout validation was supported by customer review data. |
| Cellular Localization | Endoplasmic reticulum lumen , Melanosome |
Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Grp78 antibody [N2C1], Internal detects Grp78 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody [N2C1], Internal diluted at 1:100. Blue: Hoechst 33342 staining.
Application
Immunoprecipitation of Grp78 protein from HepG2 whole cell extracts using 5 μg of Grp78 antibody [N2C1], Internal . Western blot analysis was performed using Grp78 antibody [N2C1], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Grp78 antibody [N2C1], Internal dilution: 1:50.
Application
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Grp78 antibody [N2C1], Internal dilution: 1:50.
Application
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Grp78 antibody [N2C1], Internal diluted at 1:50.
Application
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. Grp78 antibody [N2C1], Internal diluted at 1:50.
Application
Grp78 antibody detects Grp78 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at a dilution of 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 |
|---|---|
| Application Notes | WB:1:100~1:400 IP:1:20~1:100 ICC/IF:1:20~1:100 IHC-p:1:10~1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
