Application
GRP94 antibody detects GRP94 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody diluted by 1:1000Application
GRP94 antibody detects GRP94 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. GRP94 antibody diluted at 1:500.Application
Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody . Western blot analysis was performed using GRP94 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
GRP94 antibody detects GRP94 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody diluted by 1:1000Application
GRP94 antibody detects GRP94 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GRP94 protein stained by GRP94 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GRP94 antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2500.Application
Immunohistochemical analysis of paraffin-embedded HEP3B xenograft, using GRP94 antibody at 1:100 dilution.| Product Name | GRP94 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GRP94 (heat shock protein 90kDa beta (Grp94), member 1)HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human GRP94. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineDog (95%), Pig (95%), Chicken (80%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ECGP antibody GP96 antibody GRP94 antibody TRA1 antibody HSP90B1 antibody endothelial cell (HBMEC) glycoprotein antibody tumor rejection antigen 1 antibody GRP-94 antibody tumor rejection antigen (gp96) 1 antibody Tumor rejection antigen-1 (gp96) |
|---|---|
| Molecular Weight(MW) | 92 kDa (note) |
| Cellular Localization | Endoplasmic reticulum lumen , Melanosome |
Application
GRP94 antibody detects GRP94 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody diluted by 1:1000
Application
GRP94 antibody detects GRP94 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. GRP94 antibody diluted at 1:500.
Application
Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody . Western blot analysis was performed using GRP94 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
GRP94 antibody detects GRP94 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody diluted by 1:1000
Application
GRP94 antibody detects GRP94 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GRP94 protein stained by GRP94 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GRP94 antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2500.
Application
Immunohistochemical analysis of paraffin-embedded HEP3B xenograft, using GRP94 antibody at 1:100 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 , Mouse brain , PC-12 , Rat-2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
