Application
Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody [N1N3] . Western blot analysis was performed using GRP94 antibody [N1N3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
GRP94 antibody [N1N3] detects GRP94 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GRP94 protein stained by GRP94 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:5000Application
Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft , using HSP90B antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela S3 7.5% SDS PAGE diluted at 1:1000| Product Name | GRP94 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GRP94 (heat shock protein 90kDa beta (Grp94), member 1)HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GRP94. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Dog, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineRat (97%), Zebrafish (84%), Xenopus laevis (84%), Dog (99%), Pig (99%), Chicken (92%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ECGP antibody GP96 antibody GRP94 antibody TRA1 antibody HSP90B1 antibody endothelial cell (HBMEC) glycoprotein antibody tumor rejection antigen 1 antibody GRP-94 antibody tumor rejection antigen (gp96) 1 antibody Tumor rejection antigen-1 (gp96) |
|---|---|
| Molecular Weight(MW) | 92 kDa (note) |
| Cellular Localization | Endoplasmic reticulum lumen , Melanosome |
Application
Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody [N1N3] . Western blot analysis was performed using GRP94 antibody [N1N3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
GRP94 antibody [N1N3] detects GRP94 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GRP94 protein stained by GRP94 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:5000
Application
Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft , using HSP90B antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela S3 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , *endothelial cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
