Application
The cell lysates of hES(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Application
Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)| Product Name | GRP94 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2H3] to TRA1Tumor rejection antigen 1(TRA1) is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA1 is a marker protein of these stem cells. |
| Immunogen | Recombinant human TRA1 (676-803aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2H3 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| Immunofluorescence: Use at 1:100 ~ 1:200.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 94 kDa glucoseregulated protein94 kDa glucose regulated prote GRP 9 GRP9 94 kDa glucoseregulated protein 94 kDa glucose-regulated protein 94 kDa glucose regulated prote 94 kDa glucose-regulated protein GP96 94 kDa glucoseregulated protein94 kDa glu |
|---|---|
| Target | TRA1 |
| Cellular Localization | Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Light Chain Type | kappa |
Application
The cell lysates of hES(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Application
Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)| Application Notes | Immunofluorescence: Use at 1:100 ~ 1:200.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
