Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3 alpha (Phospho-Ser21) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells untreated (lane 1) or treated with serum (lane 2) using GSK3 alpha (Phospho-Ser21) Antibody .Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3a (Phospho-Ser21) Antibody| Product Name | GSK3 alpha (phospho Ser21) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GSK3 alpha (Phospho Ser21)Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. |
| Immunogen | Peptide sequence around phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3 alpha . |
| Modification | p-Ser21 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686D0638 GSK3A glycogen synthase kinase-3 alpha serine/threonine-protein kinase GSK3A GSK-3 alpha glycogen synthase kinase 3 alpha |
|---|---|
| Target | GSK3 alpha (Phospho Ser21) |
| Molecular Weight(MW) | 51 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3 alpha (Phospho-Ser21) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells untreated (lane 1) or treated with serum (lane 2) using GSK3 alpha (Phospho-Ser21) Antibody .
Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3a (Phospho-Ser21) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
