Application
GSPT1 antibody detects GSPT protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSPT protein stained by GSPT1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
GSPT1 antibody detects GSPT1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using GSPT1 antibody at 1:500 dilution.| Product Name | GSPT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GSPT1 (eukaryotic peptide chain release factor GTP-binding subunit ERF3A) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GSPT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (89%), Rat (89%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 551G9.2 antibody ETF3A antibody FLJ38048 antibody FLJ39067 antibody GST1 antibody eRF3a antibody GSPT1 antibody eukaryotic peptide chain release factor subunit 3a antibody eukaryotic peptide chain release factor GTP-binding subunit ERF3A antibody |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (2) |
Application
GSPT1 antibody detects GSPT protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSPT protein stained by GSPT1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
GSPT1 antibody detects GSPT1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using GSPT1 antibody at 1:500 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
