Application
GST-tagged protein (Mw: 45 kDa) was immunoprecipitated from E. coli lysate using 8 ug of anti-GST antibody . The precipitated protein was detected by diluted at 1:3000.
Application
Sample A: 20μg of E. coli only B: 20μg of Transformed E. coli C: 20μg of Transformed E. coli and IPTG induced12% SDS PAGE diluated at 1:3000