Application
Recombinant GST-PIN1 was separated by SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 0.5μg/ml for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: 100ng Lane 2: 50ng Lane 3: 25ng Lane 4: 10ng Lane 5: 5ng Lane 6: 2ng Lane 7: 1ng| Product Name | GST antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal [BDI340] to GST |
| Immunogen | Recombinant GST (pGEX), Schistosoma japonicum |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | BDI340 |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| ELISA: Use at a concentration of 0.05 μg. IP: Use at a concentration of 1 - 10 μg. WB: Use at 1ug/ml for AP/BCIP/NBT (MTT) detection 0.1ug/ml for HRPO/ECL detection.Predicted molecular weight: 25kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | GST |
|---|---|
| Target | GST |
| Tissue Specificity | Specifically interacts with GST of Schistosoma japonicum that is encoded by the pGEX expression vectors. |
| Cellular Localization | Cytoplasmic and Nuclear |
| Light Chain Type | kappa |
Application
Recombinant GST-PIN1 was separated by SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 0.5μg/ml for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: 100ng Lane 2: 50ng Lane 3: 25ng Lane 4: 10ng Lane 5: 5ng Lane 6: 2ng Lane 7: 1ng| Application Notes | ELISA: Use at a concentration of 0.05 μg. IP: Use at a concentration of 1 - 10 μg. WB: Use at 1ug/ml for AP/BCIP/NBT (MTT) detection 0.1ug/ml for HRPO/ECL detection.Predicted molecular weight: 25kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide. Constituents: PBS, Polyethylene glycol, Sucrose |
