Application
Isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli.Western blot was immunostained by anti-GST (S-tag-05). Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose. Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02)| Product Name | GST antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse Monoclonal antibody [S-tag-05] to GSTThe GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins. |
| Immunogen | GST fusion protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | S-tag-05 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| WB: Use at 1 µg/mL. Positive Control: GST fusion protein. Note: Reducing conditions: |
|
| Concentration | 1mg/ml |
| Gene Synonyms | Glutathione-S-Transferase GST Tag |
|---|---|
| Target | GST |
| Tissue Specificity | The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum . This purified antibody is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli , yeast, mammalian, and in vitro transcription/translation systems. |
Application
Isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli.Western blot was immunostained by anti-GST (S-tag-05). Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose. Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02)| Application Notes | WB: Use at 1 µg/mL. Positive Control: GST fusion protein. Note: Reducing conditions: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
