Application
Immunofluorescence analysis of methanol-fixed Hep G2, using GSTA2 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human stomach, using GSTA2 antibody at 1:100 dilution.Application
Sample(30 µg of whole cell lysate) A:Hep G2 12% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | GSTA2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GSTA2 (glutathione S-transferase alpha 2)Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GSTA2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Pig, Rhesus MonkeyPig (82%), Rhesus Monkey (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GST2 antibody GSTA2-2 antibody GTA2 antibody GTH2 antibody MGC10525 antibody GSTA2 antibody glutathione S-aralkyltransferase A2 antibody glutathione S-aryltransferase A2 antibody GST HA subunit 2 antibody glutathione S-alkyltransferase A2 antibody |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed Hep G2, using GSTA2 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human stomach, using GSTA2 antibody at 1:100 dilution.
Application
Sample(30 µg of whole cell lysate) A:Hep G2 12% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
