Application
GSTP1 antibody detects GSTP1 protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. GSTP1 antibody dilution: 1:500.Application
GSTP1 antibody detects GSTP1 protein at Cytoplasm+Nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min. Green: GSTP1 protein stained by GSTP1 antibody diluted at 1:200. Blue: Hoechst 33343 staining.Application
GSTP1 antibody detects GSTP1 protein by western blot analysis. A. 50 µg Rat brain lysate/extract 12% SDS-PAGE GSTP1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: HeLa B: Molt-4 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | GSTP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GSTP1 (glutathione S-transferase pi 1)Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GSTP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Rhesus Monkey, BovineDog (86%), Rhesus Monkey (97%), Bovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DFN7 antibody FAEES3 antibody GST3 antibody GSTP antibody PI antibody GSTP1 antibody deafness X-linked 7 antibody fatty acid ethyl ester synthase III antibody GST class-pi antibody GSTP1-1 antibody glutathione S-transferase P antibody glutathio |
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| Molecular Weight(MW) | 23 kDa (note) |
Application
GSTP1 antibody detects GSTP1 protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. GSTP1 antibody dilution: 1:500.
Application
GSTP1 antibody detects GSTP1 protein at Cytoplasm+Nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min. Green: GSTP1 protein stained by GSTP1 antibody diluted at 1:200. Blue: Hoechst 33343 staining.
Application
GSTP1 antibody detects GSTP1 protein by western blot analysis. A. 50 µg Rat brain lysate/extract 12% SDS-PAGE GSTP1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: HeLa B: Molt-4 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , Molt-4 , mouse brain , Rat brain |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
