Application
Flow cytometric Analysis of Hela cells, using anti-GSTT2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Anti-GSTT2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GSTT2Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSTT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSTT2.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GSTT2 monoclonal antibody.Application
HEK293T cells transfected with either RC200040 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GSTT2 antibody, and then analyzed by flow cytometry.| Product Name | GSTT2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [8A10] to GSTT2Glutathione S-transferase (GSTs) theta 2 (GSTT2) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: Alpha, Mu, Pi, Theta, and Zeta. The theta class members GSTT1 and GSTT2 share 55% amino acid sequence identity and both are thought to have an important role in human carcinogenesis. The theta genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq] |
| Immunogen | Full length human recombinant protein of human GSTT2 (NP_000845) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8A10 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GSTT2 MGC182032 GSTT2B |
|---|---|
| Target | GSTT2 |
| Tissue Specificity | is specific for human GSTT2 (NP_000845). |
Application
Flow cytometric Analysis of Hela cells, using anti-GSTT2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Anti-GSTT2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GSTT2
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSTT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSTT2.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GSTT2 monoclonal antibody.
Application
HEK293T cells transfected with either RC200040 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GSTT2 antibody, and then analyzed by flow cytometry.| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
