Application
Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA tag antibody at 1:500 dilution.Application
HA tag antibody detects HA tag protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA-human AKT-transfected 293T cells 7.5% SDS-PAGE HA tag antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
An ELISA plate is coated with 50 μL of a DDDDK Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK Tag antibody at concentration ranged from 3.1 to 200 ng/mL.Application
Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 μg of HA tag antibody . Western blot analysis was performed using HA tag antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | HA tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Rabbit Polyclonal antibody to HA tag |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIPWBIB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HA tag antibody HA antibody HA epitope tag antibody |
|---|
Application
Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA tag antibody at 1:500 dilution.
Application
HA tag antibody detects HA tag protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA-human AKT-transfected 293T cells 7.5% SDS-PAGE HA tag antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
An ELISA plate is coated with 50 μL of a DDDDK Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK Tag antibody at concentration ranged from 3.1 to 200 ng/mL.
Application
Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 μg of HA tag antibody . Western blot analysis was performed using HA tag antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | HA-human AKT-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
