Application
HA tag antibody [GT423] detects HA tag protein by western blot analysis. HA tag-transfected and GST tag-transfected (30 µg) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
HA Tag antibody [GT423] detects HA Tag protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA-tagged protein expressing HeLa cells 7.5% SDS-PAGE HA Tag antibody [GT423] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
HA tag antibody [GT423] detects HA-recombinant protein by immunofluorescent analysis. Sample: HA tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA-recombinant protein stained by HA tag antibody [GT423] diluted at 1:2000. Blue: Hoechst 33342 staining.Application
HA tag antibody [GT423] immunoprecipitates HA-HIF-1α protein in IP experiments. IP Sample: 400 μg cell lysate/extract of HA-HIF-1α transfected 293T cells A. Control with 2 μg of preimmune mouse IgG B. Immunoprecipitation of HA-fused protein by 2 μg of HA tag antibody [GT423] 5% SDS-PAGE The immunoprecipitated HA-HIF1α protein was detected by HIF-1α antibody diluted at 1:1000.| Product Name | HA tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse Monoclonal antibody [GT423] to HA tag |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT423 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HA tag antibody HA antibody HA epitope tag antibody |
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Application
HA tag antibody [GT423] detects HA tag protein by western blot analysis. HA tag-transfected and GST tag-transfected (30 µg) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
HA Tag antibody [GT423] detects HA Tag protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA-tagged protein expressing HeLa cells 7.5% SDS-PAGE HA Tag antibody [GT423] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
HA tag antibody [GT423] detects HA-recombinant protein by immunofluorescent analysis. Sample: HA tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA-recombinant protein stained by HA tag antibody [GT423] diluted at 1:2000. Blue: Hoechst 33342 staining.
Application
HA tag antibody [GT423] immunoprecipitates HA-HIF-1α protein in IP experiments. IP Sample: 400 μg cell lysate/extract of HA-HIF-1α transfected 293T cells A. Control with 2 μg of preimmune mouse IgG B. Immunoprecipitation of HA-fused protein by 2 μg of HA tag antibody [GT423] 5% SDS-PAGE The immunoprecipitated HA-HIF1α protein was detected by HIF-1α antibody diluted at 1:1000.| Positive Control | HeLa , HA-tagged protein expressing HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
