Application
HA tag antibody [GT4810] immunoprecipitates HA tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of HA tagged protein by 2.5 μg of HA tagg antibody 10% SDS-PAGE The immunoprecipitated HA tagged protein was detected by HA tag antibody [GT4810] diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.Application
HA tag antibody [GT4810] detects HA-recombinant protein by immunofluorescent analysis. Sample: HA tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: HA-recombinant protein stained by HA tag antibody [GT4810] diluted at 1:500. Blue: Hoechst 33342 staining.Application
HA tag antibody [GT4810] detects HA tag protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA tagged protein expressing HeLa cells 5% SDS-PAGE HA tag antibody [GT4810] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | HA tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse Monoclonal antibody [GT4810] to HA tag |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT4810 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HA tag antibody HA antibody HA epitope tag antibody |
|---|
Application
HA tag antibody [GT4810] immunoprecipitates HA tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of HA tagged protein by 2.5 μg of HA tagg antibody 10% SDS-PAGE The immunoprecipitated HA tagged protein was detected by HA tag antibody [GT4810] diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.
Application
HA tag antibody [GT4810] detects HA-recombinant protein by immunofluorescent analysis. Sample: HA tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: HA-recombinant protein stained by HA tag antibody [GT4810] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
HA tag antibody [GT4810] detects HA tag protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA tagged protein expressing HeLa cells 5% SDS-PAGE HA tag antibody [GT4810] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | HA-tagged protein expressing HeLa cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
