Application
IHC Image using - Detection of Hairy Cell Leukemia by IHC in Lymph Node| Product Name | Hairy Cell Leukemia antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DBA.44] to Hairy Cell Leukemia |
| Immunogen | Deau cell line established from a large cell lymphoma |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | DBA.44 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-FIHC-P |
| For IHC: This antibody may be diluted to a titer of 1:15-1:30 in an ABC method. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Enzymatic antigen retrieval of formalin fixed paraffin allembedded tissue sections is required prior to immunostaining. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Target | Hairy Cell Leukemia |
|---|---|
| Tissue Specificity | This antibody reacts with a subset of B lymphocytes localized in the follicular mantle zone. It reacts with 97% of hairy cell leukemia cases. This antibody shows strong positive staining in about 35% of high grade B cell lymphoma cases. |
| Cellular Localization | Cell membrane/cytoplasmic |
| Light Chain Type | kappa |
Application
IHC Image using - Detection of Hairy Cell Leukemia by IHC in Lymph Node| Positive Control | Tonsil |
|---|---|
| Application Notes | For IHC: This antibody may be diluted to a titer of 1:15-1:30 in an ABC method. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Enzymatic antigen retrieval of formalin fixed paraffin allembedded tissue sections is required prior to immunostaining. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Tissue culture supernatant containing sodium azide |
