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DID: Use at a dilution of 1/16. Using an Ouchterlony Double Diffusion (ODD) assay, in 1% agarose, 5 μl of serially diluted reconstituted antiserum is reacted against 5 μl of a 1 mg/ml solution of purified human haptoglobin (well separation: 7.5mm center to center). Titer is equivalent to the highest dilution of antiserum resulting in a visible preciptate after 24 hours. Optimal dilutions/concentrations should be determined by the end user.:
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