Application
Immunohistochemical analysis of paraffin-embedded human colon cancer(left) and ancreas cancer(right), showing cytoplasmic localization using anti-HCK antibody with DAB staining.Application
Western blot analysis using anti-HCK monoclonal antiobdy against truncated HCK recombinant protein(1) and full-length HCK-GFP transfected CHO-K1 cell lysate(2).| Product Name | HCK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3D12E10] to HCKThe protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010] |
| Immunogen | The immunogen corresponding to HCK expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3D12E10 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JTK9 P61HCK P59HCK |
|---|---|
| Target | HCK |
| Cellular Localization | 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated. |
Application
Immunohistochemical analysis of paraffin-embedded human colon cancer(left) and ancreas cancer(right), showing cytoplasmic localization using anti-HCK antibody with DAB staining.
Application
Western blot analysis using anti-HCK monoclonal antiobdy against truncated HCK recombinant protein(1) and full-length HCK-GFP transfected CHO-K1 cell lysate(2).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
